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生物物理学报&2011,&27(12)&998-1007&DOI:
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膜蛋白结晶方法论述
范俊萍, 张凯
中国科学院生物物理研究所,北京100101
膜蛋白在细胞生命活动中承担各种重要功能,其基因数目约占整个基因组编码基因的30%,但已知的膜蛋白结构数目却只占整个蛋白质结构数据库的1%左右。近年来,膜蛋白逐渐成为结构生物学的研究热点,膜蛋白结构研究在重组蛋白表达、纯化和结晶等方向也出现了各种技术上的进步。本文简要论述了目前各种与膜蛋白结构研究相关的X-射线晶体学实验方法,主要包括膜蛋白样品制备、结晶方法等。膜蛋白结构研究是机遇与挑战并存的领域,随着经验的积累和技术的发展,这一领域正在吸引着越来越多的结构生物学工作者。
Methods of Crystallography Study on Membrane Proteins
FAN Junping, ZHANG Xuejun
Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
Membrane proteins play key roles in many aspects of cell biology and account for nearly 30% of open reading frames in all known genomes. However, membrane proteins of known three dimensional structures composed only of about 1% of the current Protein Data Bank. Recent years, membrane proteins become a hot spot of the structural biology and have made considerable progress in recombinant expression, purification, and crystallization. In this review, we discuss methods that related to X-ray crystallography study on membrane proteins, including sample preparation and crystallization methods. Membrane protein structural biology is a rapidly developing research field full of challenges and opportunities and is attracting more and more structural biologists.
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DOI: 10.3724/SP.J.998
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通讯作者: 张凯,电话：(010),E-mail：zhangc@
作者Email: zhangc@
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生物物理学报&2011,&27(2)&99-107&DOI:
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灵长类镜像神经系统研究的最新进展
浙江大学心理与行为科学系、物理系交叉学科实验室，杭州 310028
近年来，镜像神经系统成为认知神经科学的研究热点。神经生理学和脑成像的相关研究表明，镜像神经系统以运动为基础，统一了动作观察和动作执行的神经机制，为理解他人动作提供了来自&内部&的支持。镜像神经系统为个体间的自然交流提供了神经基础，具有深远的进化意义。它不仅存在于灵长类，甚至在进化距离较远的物种(如沼雀和斑雀)中也有发现。作者在综述最新相关研究的基础上，分别介绍了猴和人类镜像神经系统的生理基础和认知功能，并对前人实验中的遗留问题做了总结和展望。
Advances in Studies of Primate Mirror Neuron System
Department of Psychology and Behavioral Sciences, Bio-X Laboratory in Department of Physics, Zhejiang University, Hangzhou 310028, China
Mirror neuron system has recently attracted more and more interests in the field of cognitive neuroscience. Studies using neurophysiological and brain-imaging techniques have suggested that such motor-based system provides a basic mechanism that unifies action execution and action perception. This mechanism comprises a neural basis for understanding goal-orientated actions of others from the inside. Mirror neuron system seems to be a primary and natural way whereby individuals communicate with one another. It is found not only in primates, but also in evolutionarily distant species, such as swamp sparrows and zebra finches. This paper reviews the latest progress about the mirror neuron system and discusses its physiologic basis and cognitive function in primates and human beings in detail. Finally, several questions for future research derived from previous studies have also been suggested.
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国家自然科学基金项目()，中央高校基本科研业务费专项资金
通讯作者: 姚远，电话：(1，E-mail：lisayuan@
作者Email: lisayuan@
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