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Cellular localization of adenylate cyclases activated by histamine and prostaglandin e 2 in rat gastric mucosa and their role in regulation of gastric secretion
Tairov M.M.; Bersimbaev R.I.; Argutinskaya S.V.; Salganik R.I., 1983: Cellular localization of adenylate cyclases activated by histamine and prostaglandin e 2 in rat gastric mucosa and their role in regulation of gastric secretion. Biokhimiya 48(6):
Cimetidine, the inhibitor of H2-receptors of histamine, does not affect the activation of adenylate cyclase by prostaglandin E2 (PGE2) in rat gastric mucosa homogenate. At the same time cimetidine completely inhibits the stimulating effect of histamine on adenylate cyclase activity. Using isolated rat gastric cells separated into fractions rich (to 90%) and poor (up to 25%) of parietal cells, it was demonstrated that prostaglandin-stimulated adenylate cyclase is localized in nonparietal (presumably mucoid) cells, while histamine-sensitive one is localized in parietal acid-producing cells. The mechanisms of histamine and PGE2 action are discussed and a scheme of paticipation of these substances in the regulation of gastric secretion is proposed.
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